Cell-free prediction of protein expression costs for growing cells

Translating heterologous proteins places significant burden on host cells, consuming expression resources leading to slower cell growth and productivity. Yet predicting the cost of protein production for any given gene is a major challenge, as multiple processes and factors combine to determine translation efficiency. To enable prediction of the cost of gene expression in bacteria, we describe here a standard cell-free lysate assay that provides a relative measure of resource consumption when a protein coding sequence is expressed. These lysate measurements can then be used with a computational model of translation to predict the in vivo burden placed on growing E. coli cells for a variety of proteins of different functions and lengths. Using this approach, we can predict the burden of expressing multigene operons of different designs and differentiate between the fraction of burden related to gene expression compared to action of a metabolic pathway

Carbon budget and national gross domestic product in the framework of the Paris Climate Agreement

In 2015 an unprecedented effort was made in Paris by the countries adhering to the United Nations Framework Convention on Climate Change to decrease the CO2 emissions due to the close relationships of greenhouse gases with global warming. Under the previous Kyoto Protocol, only advanced countries were committed to reduce greenhouse emissions while under the Paris Climate Agreement all countries were committed to fight against global warming. The urgency of real action has been prompted by extreme events like bushfires, heatwaves, and the ongoing pandemic. Given the strong commitments, it looks interesting, seen that all countries are involved, to verify if any sustainability pattern is evident. Our approach is encouraging, as the downward emission trend shows a high increase in sustainability between 2027 and 2037. Without exacerbating the climate discussion, we used the national gross domestic product (hereafter: GDP) as environmental indicator to propose for the first time an allometric ranking of countries that need to change drastically their energy policy to meet their climate commitments. Any sustainability downturn in one country, especially if advanced, might rationally bring concern about the actual prospects of other countries which are all committed to the Paris Climate Agreement. But the departure from the annual allometric model GDP−CO2 may be much greater than can be accounted for by statistical expectations, as for Ukraine, Uzbekistan and Turkmenistan that are entering a sustainable condition where their CO2 emissions will be lower than they would have been without the Paris Climate Agreement

Non-uniform interpolatory subdivision schemes with improved smoothness

Subdivision schemes are used to generate smooth curves or surfaces by iteratively refining an initial control polygon or mesh. We focus on univariate, linear, binary subdivision schemes, where the vertices of the refined polygon are computed as linear combinations of the current neighbouring vertices. In the classical stationary setting, there are just two such subdivision rules, which are used throughout all subdivision steps to construct the new vertices with even and odd indices, respectively. These schemes are well understood and many tools have been developed for deriving their properties, including the smoothness of the limit curves. For non-stationary schemes, the subdivision rules are not fixed and can be different in each subdivision step. Non-uniform schemes are even more general, as they allow the subdivision rules to be different for every new vertex that is generated by the scheme. The properties of non-stationary and non-uniform schemes are usually derived by relating the scheme to a corresponding stationary scheme and then exploiting the fact that the properties of the stationary scheme carry over under certain proximity conditions. In particular, this approach can be used to show that the limit curves of a non-stationary or non-uniform scheme are as smooth as those of a corresponding stationary scheme. In this paper we show that non-uniform subdivision schemes have the potential to generate limit curves that are smoother than those of stationary schemes with the same support size of the subdivision rule. For that, we derive interpolatory 2-point and 4-point schemes that generate C-1 and C-2 limit curves, respectively. These values of smoothness exceed the smoothness of classical interpolating schemes with the same support size by one. (C) 2022 The Author(s). Published by Elsevier B.V

The Effect of Added Weight on Foot Anthropometry in Pregnant Women and Controls

Please refer to the pdf version of the abstract located adjacent to the title

Crossbreeding to improve local chicken breeds: predicting growth performance of the crosses using the Gompertz model and estimated heterosis

The present study aims to validate the Gompertz model to predict the growth performance of chicken crosses according to growth curve parameters of the parental lines and the estimated heterosis for each curve parameter. A total of 252 one-day-old chicks of both sexes belonging to 6 genotypes, including Ross 308, Sasso (SA), Bionda Piemontese (BP), and Robusta Maculata (RM), and the crosses between these local breeds and SA (BP x SA and RM x SA) were randomly allocated in 18 pens (3 pens/genotype) in mixed-sex groups (14 animals/pen; 7 females and 7 males). The individual body weight (BW) of all birds was recorded once a week from hatching until slaughtering (81 d for Ross 308; 112 d for SA, 140 d for the other genotypes). We drew up our final dataset with 240 birds (40 birds/ genotype; 20 females and 20 males). The growth curve of each genotype was described using the Gompertz model, and the heterosis for each growth curve parameter was calculated as the difference between F-1 crosses and the average of parental breeds. The predicted growth curve parameters were evaluated by cross-validation. The Gompertz model accurately estimated the growth curves of all the genotypes (R-2 > 0.90). Heterosis was significant for almost all growth curve parameters in both crosses (P < 0.05). Heterosis ranged from -13.0 to +11.5%, depending on parameters, but varied slightly between the crossbreeds (BP x SA and RM x SA). The predicted values of adult BW, weight at the inflection point, and maximum growth rate were overestimated for BP x SA and under-estimated for RM x SA, with a mean error between observed and predicted values <12.71% for all the curve parameters. In conclusion, the growth performance of chicken crosses between local breeds and commercial strains can be accurately predicted with Gompertz parameters of the parental lines adjusting for heterosis

Tiny percutaneous needle biopsy: An efficient methodfor studying cellular and molecular aspectsof skeletal muscle in humans

Needle biopsy is widely used to obtain specimens for physiological, anatomical and biochemical studies of skeletal muscle (SM). We optimized a procedure which we termed tiny percutaneous needle biopsy (TPNB), to efficiently gather good numbers of human satellite cells and single dissociated fibers for the functional study of skeletal muscle; these samples permit isolation of high-quality RNA and sufficient amounts of proteins to allow molecular analysis. Moreover, TPNB showed a clear advantage in that the technique was easier than other procedures used on healthy volunteers in human trials. TPNB is a very safe minor surgical procedure. It is less traumatic than needle aspiration biopsy, and significant complications are improbable. TPNB should become established as an important tool in the investigation of SM and may be employed to study various physiological aspects of SM in human subjects. We suggest that TPNB should also be used in the study of muscle diseases and disorders including muscular dystrophy, congenital myopathy, and metabolic defects

Histamine stimulates the proliferation of small and large cholangiocytes by activation of both IP3/Ca2+ and cAMP-dependent signaling mechanisms

Although large cholangiocytes exert their functions by activation of cyclic adenosine 3',5'-monophosphate (cAMP), Ca(2+)-dependent signaling regulates the function of small cholangiocytes. Histamine interacts with four receptors, H1-H4HRs. H1HR acts by Gαq activating IP(3)/Ca(2+), whereas H2HR activates Gα(s) stimulating cAMP. We hypothesize that histamine increases biliary growth by activating H1HR on small and H2HR on large cholangiocytes. The expression of H1-H4HRs was evaluated in liver sections, isolated and cultured (normal rat intrahepatic cholangiocyte culture (NRIC)) cholangiocytes. In vivo, normal rats were treated with histamine or H1-H4HR agonists for 1 week. We evaluated: (1) intrahepatic bile duct mass (IBDM); (2) the effects of histamine, H1HR or H2HR agonists on NRIC proliferation, IP(3) and cAMP levels and PKCα and protein kinase A (PKA) phosphorylation; and (3) PKCα silencing on H1HR-stimulated NRIC proliferation. Small and large cholangiocytes express H1-H4HRs. Histamine and the H1HR agonist increased small IBDM, whereas histamine and the H2HR agonist increased large IBDM. H1HR agonists stimulated IP(3) levels, as well as PKCα phosphorylation and NRIC proliferation, whereas H2HR agonists increased cAMP levels, as well as PKA phosphorylation and NRIC proliferation. The H1HR agonist did not increase proliferation in PKCα siRNA-transfected NRICs. The activation of differential signaling mechanisms targeting small and large cholangiocytes is important for repopulation of the biliary epithelium during pathologies affecting different-sized bile ducts

Distribution and consistency of Ancona and Livorno poultry breed in Central Italy

Nowadays most of the Italian chickens’ breeds are replaced with commercial hybrids characterised by higher productive performance. However, the safeguard of biodiversity and consequently the genetic variability are a very important issue, and for this reason, it is fundamental to include local strains in preservation programs. In Italy, the poultry Genealogic Book has been recently constituted, and includes 22 chicken breeds. The diffusion of these strains is strictly linked to the territory of origin, in particular in the Central Italy, the Ancona and Livorno chicken breeds are the mainly widespread ones. The aim of this study was to determine the consistency and the characteristics of the farms involved in the farming of Ancona and Livorno chicken breeds in Central Italy. A survey to analyse the facilities, the human resources and the chicken population consistencies was conducted in the farms of Abruzzo, Lazio, Marche, Tuscany and Umbria regions. All the investigated farms can be identified as ‘hobby-farms’ being small scale ones and leading the breeding of Ancona and Livorno chickens as a secondary business. The survey also confirmed that the presence of the strains depends on the region: 100% of Marche farms bred Ancona whereas, in Tuscany the Livorno breed is the most common.Highlights A survey in centre Italy was carried out to determine the consistency of Ancona and Livorno chicken breeds; The Ancona and Livorno chickens breed were reared in small scale farms; The widespread of the breeds depend on the region: Ancona breed is most present in the Marche region while the Livorno is mainly bred in Tuscany